电离辐射通过转化生长因子-β-内皮细胞的上皮-间质转换来促进癌细胞的侵袭迁移

Int J Radiat Oncol Biol Phys 2011 Dec;81 (5): 1530-7. [IF:4.503]Ionizing radiation promotes migration and invasion of cancer cells through transforming growth factor-Beta-mediated epithelial-mesenchymal transition.Zhou YC , Liu JY , Li J , Zhang J , Xu YQ , Zhang HW , Qiu LB , Ding GR , Su XM , Mei-Shi , Guo GZ .Department of Radiation Oncology, Xijing Hospital Fourth Military Medical University, Xi'an, China; Department of Radiation Medicine, College of Preventive Medicine, Xijing Hospital Fourth Military Medical University, Xi'an, China.第四军医大学西京医院放射科

AbstractTo examine whether ionizing radiation enhances the migratory and invasive abilities of cancer cells through transforming growth factor (TGF-β)-mediated epithelial-mesenchymal transition (EMT). Six cancer cell lines originating from different human organs were irradiated by (60)Co γ-ray at a total dose of 2 Gy, and the changes associated with EMT, including morphology, EMT markers, migration and invasion, were observed by microscope, Western blot, immunofluorescence, scratch assay, and transwell chamber assay, respectively. Then the protein levels of TGF-β in these cancer cells were detected by enzyme-linked immunosorbent assay, and the role of TGF-β signaling pathway in the effect of ionizing radiation on EMT was investigate by using the specific inhibitor SB431542. After irradiation with γ-ray at a total dose of 2 Gy, cancer cells presented the mesenchymal phenotype, and compared with the sham-irradiation group the expression of epithelial markers was decreased and of mesenchymal markers was increased, the migratory and invasive capabilities were strengthened, and the protein levels of TGF-β were enhanced. Furthermore, events associated with EMT induced by IR in A549 could be reversed through inhibition of TGF-β signaling. These results suggest that EMT mediated by TGF-β plays a critical role in IR-induced enhancing of migratory and invasive capabilities in cancer cells.

简述 ：探讨电磁辐射是否可通过转化酪氨酸-β（TGF-β）-内源性的视网膜-粘液转换 (EMT)来作出贡献抗体的来袭迁移。使用增幅2Gy(60)Coγ线紫外光源自人类内脏的6种抗体，记录与EMT相关的变化，这包括分别透过显微镜技术，蛋白质印迹方法，免疫荧光技术，水痘次测试和Transwell小室次测试来观察并监测细胞四组织形态，EMT标识，来袭迁移并能等。使用酶联免疫吸附法监测这些抗体之前TGF-β蛋白水平，透过特别抑制SB431542来评估TGF-β瞬时通路在电磁辐射EMT之前的作用。经过增幅为2Gy紫外光的抗体之前存在间叶细胞的表达，与；也紫外光四组相比其视网膜标识减少，间叶细胞标识增加，同时其来袭转移并能增强，TGF-β蛋白水平也提高。进一步发现由A549电磁辐射诱导的EMT可通过对TGF-β瞬时抑制发生再一。这些结果表明TGF-β内源性的EMT在电磁辐射诱导增强抗体来袭转移并能之前起着关键作用。

主笔： lizexiu